IgG quantitation is often performed via relatively expensive and or time-consuming techniques such BLI, HPLC or ELISA
In contrast the new ValitaTiter assay from Beckman Coulter utilizes a unique reagent to measure IgG binding directly via fluorescence polarisation – An available feature on many compatible readers. ValitaTiter assay plates are provided precoated with lyophilized reagent in a ready-to-go state: simply Add, Mix, Run.
In a simplified analogy – how the fluorescence polarisation works comes down to how fast the reagent ‘spins’ determines the length of time at the correct emission orientations to the parallel or perpendicular detectors. Free probe therefore gives lower FP signal measured in mP than target bound probe, as the latter complex spins more slowly in solution.
The ValitaTiter assay is well correlated with industry standard techniques like Protein-A HPLC and BLI – however, as well as being more cost-effective in terms of cost/sample – it much more amenable to automation workflows or high-throughput studies.
Demo or trial is certainly recommended for any medium or high-throughput IgG workflows. Such as advanced R&D with IgG concentrations within either range below, Clone selection, as well as initial scale-up workflows, or QC or adjacent testing
